--- title: "Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older…" description: "GlyNAC supplementation in older adults can improve several age-related health issues, including glutathione deficiency, oxidative stress, and physical…" url: "https://magellanlongevity.com/study/glutathione_glynac.md" canonical: "https://magellanlongevity.com/study/glutathione_glynac.html" html: "https://magellanlongevity.com/study/glutathione_glynac.html" type: "study" study_key: "glutathione_glynac" journal: "The Journals of Gerontology: Series A" year: 2022 pmid: "35975308" doi: "10.1093/gerona/glac135" citations: 1 updated: 2026-09-02 author: "Gabriel Radu, DO" author_credentials: "Physiatrist (PM&R). NY medical license 275110. NPI 1376861765." publisher: "Magellan Longevity" content_format: "markdown" --- # Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Inflammation, Physical Function, and Aging Hallmarks: A Randomized Clinical Trial [Home](https://magellanlongevity.com/) › [Research map](https://magellanlongevity.com/longevity-research-map.html) › Supplementing Glycine and N-Acetylcysteine (GlyNAC) in… **HTML version:** https://magellanlongevity.com/study/glutathione_glynac.html · **In the Magellan app:** https://magellanlongevity.com/#/study/glutathione_glynac ## Citation | Field | Value | | --- | --- | | Journal | The Journals of Gerontology: Series A | | Year | 2022 | | PMID | [35975308](https://pubmed.ncbi.nlm.nih.gov/35975308/) | | DOI | [10.1093/gerona/glac135](https://doi.org/10.1093/gerona/glac135) | | Study key | glutathione_glynac | ## Takeaway GlyNAC supplementation in older adults can improve several age-related health issues, including glutathione deficiency, oxidative stress, and physical function. ## The question Can GlyNAC supplementation improve glutathione deficiency, oxidative stress, mitochondrial dysfunction, insulin resistance, physical function, and aging hallmarks in older adults? ## Hypothesis Supplementing GlyNAC in older adults will improve glutathione deficiency, oxidative stress, mitochondrial dysfunction, insulin resistance, physical function, and aging hallmarks, similar to observations in aged mice. ## Methods A placebo-controlled randomized clinical trial was conducted with 24 older adults (OA) and 12 young adults (YA). OA were randomized to receive either GlyNAC (N=12) or an isonitrogenous alanine placebo (N=12) for 16 weeks. YA (N=12) received GlyNAC for 2 weeks. Participants were assessed before, after 2 weeks, and after 16 weeks for GSH concentrations, oxidative stress, mitochondrial function, inflammation, insulin resistance, physical function, and aging hallmarks. ## Results Compared to young adults, older adults exhibited glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, insulin resistance, multiple aging hallmarks, and impaired physical function. GlyNAC supplementation in older adults, but not placebo, improved or corrected these observed defects. ## Conclusion GlyNAC supplementation for 16 weeks in older adults was safe, well-tolerated, and effectively improved and reversed multiple age-associated abnormalities, suggesting it can promote health in aging humans. ## Limitations The abstract does not explicitly state any limitations of the study. ## Verbatim from the abstract > GlyNAC supplementation in older adults improved glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, and multiple hallmarks of aging. ## Where this study is used on Magellan This paper is one of the citations behind the evidence grade on the pages below. Grades are assigned from the published research and are independent of affiliate commissions. | Compound or device | Evidence grade | Graded pages | | --- | --- | --- | | All-in-One Longevity Formulation | Emerging evidence | [NOVOS Core All-In-One Sachets](https://magellanlongevity.com/p/12.md) | | Targeted Organ-Support Nutrients | Emerging evidence | [NOVOS Vital Organ Support](https://magellanlongevity.com/p/14.md) | | Polyphenol Antioxidant Blend | Moderate evidence | [DaVinci Labs Phyto Benefits](https://magellanlongevity.com/p/19.md) | | Glutathione | Moderate evidence | [DaVinci Labs Glutathione Bright](https://magellanlongevity.com/p/20.md) | | Cellular Senescence & Inflammation Panel | — | — | | Glycan-Based Inflammatory Age Testing | — | — | | At-Home Food & Allergen Testing | Preliminary | [Allergen Alert Mini Food Lab](https://magellanlongevity.com/p/79.md) | | Glycine Powder | Moderate evidence | [Glycine Powder](https://magellanlongevity.com/p/125.md) | | GlyNAC (Glycine + N-Acetylcysteine) | Emerging evidence | [GlyNAC (Glycine + NAC)](https://magellanlongevity.com/p/198.md) | ## Reference 1. **Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Inflammation, Physical Function, and Aging Hallmarks: A Randomized Clinical Trial** The Journals of Gerontology: Series A 2022 · [PMID 35975308](https://pubmed.ncbi.nlm.nih.gov/35975308/) · [DOI 10.1093/gerona/glac135](https://doi.org/10.1093/gerona/glac135) > GlyNAC supplementation in older adults improved glutathione deficiency, oxidative stress, mitochondrial dysfunction, inflammation, and multiple hallmarks of aging. ## Structured data ```json {"@context":"https://schema.org","@graph":[{"@type":"MedicalWebPage","@id":"https://magellanlongevity.com/study/glutathione_glynac.html#page","url":"https://magellanlongevity.com/study/glutathione_glynac.html","name":"Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency,…","description":"GlyNAC supplementation in older adults can improve several age-related health issues, including glutathione deficiency, oxidative stress, and physical…","inLanguage":"en","isPartOf":{"@type":"WebSite","name":"Magellan Longevity","url":"https://magellanlongevity.com/"},"author":{"@type":"Person","name":"Gabriel Radu, DO","jobTitle":"Physiatrist (PM&R)","identifier":[{"@type":"PropertyValue","name":"NPI","value":"1376861765"},{"@type":"PropertyValue","name":"NY medical license","value":"275110"}]},"publisher":{"@type":"Organization","name":"Magellan Longevity","url":"https://magellanlongevity.com/"},"encoding":{"@type":"MediaObject","encodingFormat":"text/markdown","contentUrl":"https://magellanlongevity.com/study/glutathione_glynac.md"},"dateModified":"2026-09-02"},{"@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://magellanlongevity.com/"},{"@type":"ListItem","position":2,"name":"Research map","item":"https://magellanlongevity.com/longevity-research-map.html"},{"@type":"ListItem","position":3,"name":"Supplementing Glycine and N-Acetylcysteine (GlyNAC) in…","item":"https://magellanlongevity.com/study/glutathione_glynac.html"}]},{"@type":"ScholarlyArticle","@id":"https://magellanlongevity.com/study/glutathione_glynac.html#study","name":"Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency, Oxidative Stress, Mitochondrial Dysfunction, Inflammation, Physical Function, and Aging Hallmarks: A Randomized Clinical Trial","headline":"Supplementing Glycine and N-Acetylcysteine (GlyNAC) in Older Adults Improves Glutathione Deficiency,…","datePublished":"2022","isPartOf":{"@type":"Periodical","name":"The Journals of Gerontology: Series A"},"identifier":"PMID:35975308","url":"https://pubmed.ncbi.nlm.nih.gov/35975308/","sameAs":"https://doi.org/10.1093/gerona/glac135","abstract":"GlyNAC supplementation in older adults can improve several age-related health issues, including glutathione deficiency, oxidative stress, and physical function."}]} ``` ## Related on Magellan Longevity - 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Nothing here is intended to diagnose, treat, cure, or prevent any disease. Talk to your physician before starting any supplement or device, especially if you are pregnant, nursing, or taking medication. Editorial firewall: evidence grades are assigned from the published research and are independent of any affiliate commission. As an Amazon Associate, Magellan Longevity earns from qualifying purchases. Reviewed for accuracy by a board-certified physician (DO).