--- title: "The efficacy and safety of β-nicotinamide mononucleotide (NMN)…" description: "Daily NMN supplementation up to 900 mg is safe and increases NAD levels, physical performance, and subjective health in middle-aged adults, with 600 mg…" url: "https://magellanlongevity.com/study/nmn_trial.md" canonical: "https://magellanlongevity.com/study/nmn_trial.html" html: "https://magellanlongevity.com/study/nmn_trial.html" type: "study" study_key: "nmn_trial" journal: "GeroScience" year: 2022 pmid: "36482258" doi: "10.1007/s11357-022-00705-1" citations: 1 updated: 2026-09-02 author: "Gabriel Radu, DO" author_credentials: "Physiatrist (PM&R). NY medical license 275110. NPI 1376861765." publisher: "Magellan Longevity" content_format: "markdown" --- # The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial. [Home](https://magellanlongevity.com/) › [Research map](https://magellanlongevity.com/longevity-research-map.html) › The efficacy and safety of β-nicotinamide mononucleotide… **HTML version:** https://magellanlongevity.com/study/nmn_trial.html · **In the Magellan app:** https://magellanlongevity.com/#/study/nmn_trial ## Citation | Field | Value | | --- | --- | | Journal | GeroScience | | Year | 2022 | | PMID | [36482258](https://pubmed.ncbi.nlm.nih.gov/36482258/) | | DOI | [10.1007/s11357-022-00705-1](https://doi.org/10.1007/s11357-022-00705-1) | | Study key | nmn_trial | ## Takeaway Daily NMN supplementation up to 900 mg is safe and increases NAD levels, physical performance, and subjective health in middle-aged adults, with 600 mg showing the highest efficacy. ## The question Can β-nicotinamide mononucleotide (NMN) supplementation increase NAD concentrations and improve health in middle-aged healthy adults, and what are its safety and efficacy at different doses? ## Hypothesis NMN supplementation will increase NAD concentrations and improve healthspan parameters in humans, similar to findings in animal studies, and will be safe and well-tolerated. ## Methods This was a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial. 80 healthy middle-aged adults were randomized to receive daily oral doses of placebo, 300 mg, 600 mg, or 900 mg NMN for 60 days. Blood NAD concentration was the primary outcome, while secondary outcomes included safety, tolerability, physical performance (six-minute walking test), blood biological age, HOMA-IR, and subjective general health (SF-36). ## Results Blood NAD concentrations significantly increased in all NMN-treated groups at days 30 and 60 compared to placebo and baseline, with the highest concentrations in the 600 mg and 900 mg groups. No safety issues were observed, and NMN was well tolerated. Walking distance significantly increased in all NMN groups compared to placebo, with the longest distances in the 600 mg and 900 mg groups. Blood biological age increased in the placebo group but remained unchanged in NMN groups. HOMA-IR showed no significant differences, while SF-36 scores indicated significantly better health in all NMN groups compared to placebo. ## Conclusion NMN supplementation increases blood NAD concentrations and is safe and well tolerated at oral doses up to 900 mg daily in healthy middle-aged adults. Clinical efficacy, including improvements in physical performance and subjective health, appears highest at a dose of 600 mg daily. ## Limitations The abstract does not explicitly state any limitations of the study. ## Verbatim from the abstract > In animal studies, β-nicotinamide mononucleotide (NMN) supplementation increases nicotinamide adenine dinucleotide (NAD) concentrations and improves healthspan and lifespan with great safety. ## NMN Dose-Dependent Efficacy | Measure | NMN Dose (mg) | | --- | --- | | 300 mg NMN | 300 | | 600 mg NMN | 600 | | 900 mg NMN | 900 | *The abstract indicates that blood NAD concentrations and walking distances were highest in the 600 mg and 900 mg NMN groups, and clinical efficacy reached highest at 600 mg daily.* ## Where this study is used on Magellan This paper is one of the citations behind the evidence grade on the pages below. Grades are assigned from the published research and are independent of affiliate commissions. | Compound or device | Evidence grade | Graded pages | | --- | --- | --- | | Nicotinamide Mononucleotide (NMN) | Moderate evidence | [Double Wood NMN Capsules](https://magellanlongevity.com/p/2.md), [Nutricost NMN Capsules 500mg](https://magellanlongevity.com/p/3.md), [NOVOS Boost NMN](https://magellanlongevity.com/p/13.md) | | NAD⁺ Activation Stack (NMN + Resveratrol + Pterostilbene) | — | — | | All-in-One Longevity Formulation | Emerging evidence | [NOVOS Core All-In-One Sachets](https://magellanlongevity.com/p/12.md) | ## Reference 1. **The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial.** GeroScience 2022 · [PMID 36482258](https://pubmed.ncbi.nlm.nih.gov/36482258/) · [DOI 10.1007/s11357-022-00705-1](https://doi.org/10.1007/s11357-022-00705-1) > In animal studies, β-nicotinamide mononucleotide (NMN) supplementation increases nicotinamide adenine dinucleotide (NAD) concentrations and improves healthspan and lifespan with great safety. ## Structured data ```json {"@context":"https://schema.org","@graph":[{"@type":"MedicalWebPage","@id":"https://magellanlongevity.com/study/nmn_trial.html#page","url":"https://magellanlongevity.com/study/nmn_trial.html","name":"The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged…","description":"Daily NMN supplementation up to 900 mg is safe and increases NAD levels, physical performance, and subjective health in middle-aged adults, with 600 mg…","inLanguage":"en","isPartOf":{"@type":"WebSite","name":"Magellan Longevity","url":"https://magellanlongevity.com/"},"author":{"@type":"Person","name":"Gabriel Radu, DO","jobTitle":"Physiatrist (PM&R)","identifier":[{"@type":"PropertyValue","name":"NPI","value":"1376861765"},{"@type":"PropertyValue","name":"NY medical license","value":"275110"}]},"publisher":{"@type":"Organization","name":"Magellan Longevity","url":"https://magellanlongevity.com/"},"encoding":{"@type":"MediaObject","encodingFormat":"text/markdown","contentUrl":"https://magellanlongevity.com/study/nmn_trial.md"},"dateModified":"2026-09-02"},{"@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https://magellanlongevity.com/"},{"@type":"ListItem","position":2,"name":"Research map","item":"https://magellanlongevity.com/longevity-research-map.html"},{"@type":"ListItem","position":3,"name":"The efficacy and safety of β-nicotinamide mononucleotide…","item":"https://magellanlongevity.com/study/nmn_trial.html"}]},{"@type":"ScholarlyArticle","@id":"https://magellanlongevity.com/study/nmn_trial.html#study","name":"The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged adults: a randomized, multicenter, double-blind, placebo-controlled, parallel-group, dose-dependent clinical trial.","headline":"The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in healthy middle-aged…","datePublished":"2022","isPartOf":{"@type":"Periodical","name":"GeroScience"},"identifier":"PMID:36482258","url":"https://pubmed.ncbi.nlm.nih.gov/36482258/","sameAs":"https://doi.org/10.1007/s11357-022-00705-1","abstract":"Daily NMN supplementation up to 900 mg is safe and increases NAD levels, physical performance, and subjective health in middle-aged adults, with 600 mg showing the highest efficacy."}]} ``` ## Related on Magellan Longevity - [Double Wood NMN Capsules](https://magellanlongevity.com/p/2.md) — product graded on this and related evidence - [Nutricost NMN Capsules 500mg](https://magellanlongevity.com/p/3.md) — product graded on this and related evidence - [NOVOS Boost NMN](https://magellanlongevity.com/p/13.md) — product graded on this and related evidence - [NOVOS Core All-In-One Sachets](https://magellanlongevity.com/p/12.md) — product graded on this and related evidence - [The Safety and Antiaging Effects of Nicotinamide Mononucleotide in Human Clinical…](https://magellanlongevity.com/study/nmn_update.md) - [Caloric restriction, resveratrol and melatonin: Role of SIRT1 and implications for aging…](https://magellanlongevity.com/study/resv_sirt1.md) - [NAD metabolism and arterial stiffness after long-term nicotinamide mononucleotide…](https://magellanlongevity.com/study/x1.md) - [Towards personalized NMN supplementation: nicotinamide adenine dinucleotide (NAD)…](https://magellanlongevity.com/study/nmn_dose.md) - [Longevity research map](https://magellanlongevity.com/longevity-research-map.html) ## Scope and disclosures Educational information, not medical advice. Nothing here is intended to diagnose, treat, cure, or prevent any disease. Talk to your physician before starting any supplement or device, especially if you are pregnant, nursing, or taking medication. Editorial firewall: evidence grades are assigned from the published research and are independent of any affiliate commission. As an Amazon Associate, Magellan Longevity earns from qualifying purchases. Reviewed for accuracy by a board-certified physician (DO).