---
title: "NAD metabolism and arterial stiffness after long-term…"
description: "NMN supplementation safely increased NAD metabolism in healthy middle-aged adults and showed a non-significant trend towards reducing arterial stiffness."
url: "https://magellanlongevity.com/study/x1.md"
canonical: "https://magellanlongevity.com/study/x1.html"
html: "https://magellanlongevity.com/study/x1.html"
type: "study"
study_key: "x1"
journal: "Sci Rep"
year: 2023
pmid: "36797393"
doi: "10.1038/s41598-023-29787-3"
citations: 1
updated: 2026-09-02
author: "Gabriel Radu, DO"
author_credentials: "Physiatrist (PM&R). NY medical license 275110. NPI 1376861765."
publisher: "Magellan Longevity"
content_format: "markdown"
---

# NAD metabolism and arterial stiffness after long-term nicotinamide mononucleotide supplementation: a randomized controlled trial

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**HTML version:** https://magellanlongevity.com/study/x1.html · **In the Magellan app:** https://magellanlongevity.com/#/study/x1

## Citation

| Field | Value |
| --- | --- |
| Journal | Sci Rep |
| Year | 2023 |
| PMID | [36797393](https://pubmed.ncbi.nlm.nih.gov/36797393/) |
| DOI | [10.1038/s41598-023-29787-3](https://doi.org/10.1038/s41598-023-29787-3) |
| Study key | x1 |

## Takeaway

NMN supplementation safely increased NAD metabolism in healthy middle-aged adults and showed a non-significant trend towards reducing arterial stiffness.

## The question

Does long-term nicotinamide mononucleotide (NMN) supplementation influence biochemical and metabolic health parameters, including NAD metabolism and arterial stiffness, in healthy middle-aged adults?

## Hypothesis

The study implicitly hypothesized that NMN supplementation would prevent the reduction of NAD levels and potentially alleviate aging-related diseases, as observed in animal studies.

## Methods

A 12-week randomized, double-blind, placebo-controlled, parallel-group clinical trial was conducted with 36 healthy middle-aged participants. Participants received either 125 mg NMN or placebo twice daily.

## Results

Serum nicotinamide levels were significantly higher in the NMN group compared to the placebo group. Pulse wave velocity, an indicator of arterial stiffness, tended to decrease in the NMN group, but this difference was not statistically significant between the two groups. NMN supplementation at 250 mg/day was well tolerated with no adverse events.

## Conclusion

NMN supplementation safely and effectively elevated NAD metabolism in healthy middle-aged adults. While NMN showed potential in alleviating arterial stiffness, this effect was not statistically significant in this study.

## Limitations

The study noted that while arterial stiffness tended to decrease, no significant difference was found between the two groups, suggesting a potential limitation in statistical power or effect size for this specific outcome.

## Verbatim from the abstract

> NMN supplementation showed potential in alleviating arterial stiffness

## NMN Supplementation Dosage

| Measure | Dose (mg) |
| --- | --- |
| NMN Dose per Capsule | 125 |
| Total Daily NMN Dose | 250 |

*Participants received 125 mg of NMN per capsule, taken twice daily for a total daily dose of 250 mg.*

## Where this study is used on Magellan

This paper is one of the citations behind the evidence grade on the pages below. Grades are assigned from the published research and are independent of affiliate commissions.

| Compound or device | Evidence grade | Graded pages |
| --- | --- | --- |
| Nicotinamide Mononucleotide (NMN) | Moderate evidence | [Double Wood NMN Capsules](https://magellanlongevity.com/p/2.md), [Nutricost NMN Capsules 500mg](https://magellanlongevity.com/p/3.md), [NOVOS Boost NMN](https://magellanlongevity.com/p/13.md) |
| NAD⁺ Activation Stack (NMN + Resveratrol + Pterostilbene) | — | — |

## Reference

1. **NAD metabolism and arterial stiffness after long-term nicotinamide mononucleotide supplementation: a randomized controlled trial**
   Sci Rep 2023 · [PMID 36797393](https://pubmed.ncbi.nlm.nih.gov/36797393/) · [DOI 10.1038/s41598-023-29787-3](https://doi.org/10.1038/s41598-023-29787-3)

   > NMN supplementation showed potential in alleviating arterial stiffness

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## Related on Magellan Longevity

- [Double Wood NMN Capsules](https://magellanlongevity.com/p/2.md) — product graded on this and related evidence
- [Nutricost NMN Capsules 500mg](https://magellanlongevity.com/p/3.md) — product graded on this and related evidence
- [NOVOS Boost NMN](https://magellanlongevity.com/p/13.md) — product graded on this and related evidence
- [NMN Complex 1000 mg (Micro Ingredients)](https://magellanlongevity.com/p/1.md) — product graded on this and related evidence
- [The efficacy and safety of β-nicotinamide mononucleotide (NMN) supplementation in…](https://magellanlongevity.com/study/nmn_trial.md)
- [The Safety and Antiaging Effects of Nicotinamide Mononucleotide in Human Clinical…](https://magellanlongevity.com/study/nmn_update.md)
- [Caloric restriction, resveratrol and melatonin: Role of SIRT1 and implications for aging…](https://magellanlongevity.com/study/resv_sirt1.md)
- [Towards personalized NMN supplementation: nicotinamide adenine dinucleotide (NAD)…](https://magellanlongevity.com/study/nmn_dose.md)
- [Longevity research map](https://magellanlongevity.com/longevity-research-map.html)

## Scope and disclosures

Educational information, not medical advice. Nothing here is intended to diagnose, treat, cure, or prevent any disease. Talk to your physician before starting any supplement or device, especially if you are pregnant, nursing, or taking medication.

Editorial firewall: evidence grades are assigned from the published research and are independent of any affiliate commission. As an Amazon Associate, Magellan Longevity earns from qualifying purchases.

Reviewed for accuracy by a board-certified physician (DO).