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L-Methylfolate (5-MTHF)

Written and medically reviewed by , physiatrist · Last reviewed · How we grade
In one paragraph

L-Methylfolate (5-MTHF), graded Moderate-evidence on Magellan — evidence for hard clinical outcomes is more mixed: meta-analyses of folate supplementation show a modest (~10%) reduction in stroke risk but little effect on coronary heart disease or all-cause mortality, and B-vitamin trials have generally not slowed cognitive decline in older adults.

Source: Clin Nutr ESPEN 2023, PMID 38056998 ↗ · Nutraceuticals & Cellular Energizers · How Magellan grades evidence · Study write-up · evidence confidence: high

Nutraceuticals & Cellular Energizers21 PubMed citations
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What it is

L-Methylfolate (also called 5-methyltetrahydrofolate, 5-MTHF, or levomefolic acid) is the biologically active, circulating form of the B-vitamin folate and the form that enters cells and the brain without further conversion. Unlike synthetic folic acid, it does not require reduction by dihydrofolate reductase or activation by methylenetetrahydrofolate reductase (MTHFR), so its bioavailability is not reduced by common MTHFR gene polymorphisms. Within one-carbon metabolism it donates a methyl group to convert homocysteine into methionine, supporting DNA methylation and neurotransmitter synthesis.

Its role in longevity

In human trials, both L-5-MTHF and folic acid raise blood folate and lower total homocysteine, an amino acid whose elevation is linked to cardiovascular and cognitive risk; head-to-head studies find L-5-MTHF at least as effective as folic acid, and sometimes better, at improving folate status. Evidence for hard clinical outcomes is more mixed: meta-analyses of folate supplementation show a modest (~10%) reduction in stroke risk but little effect on coronary heart disease or all-cause mortality, and B-vitamin trials have generally not slowed cognitive decline in older adults. As an adjunct to antidepressants, 15 mg/day L-methylfolate improved response in SSRI-resistant depression in randomized trials and a 2022 meta-analysis (about 25% higher response rates, NNT around 6, tolerability like placebo), with benefit concentrated in people with obesity, inflammation or certain folate-pathway genotypes; a 7.5 mg dose was ineffective. Depression augmentation is the best-tested use — longevity-specific indications are extrapolations, not tested. This evidence describes the folate compound and homocysteine mechanism itself, not this specific commercial product.

The research

Peer-reviewed studies on the active compound — citations link to PubMed.

“serum folate increased while total homocysteine decreased significantly”
Clin Nutr ESPEN 2023 · PMID 38056998
“greater pooled mean change with L-methylfolate versus placebo”
J Clin Psychiatry 2014 · PMID 24813065
“L-MTHF was more effective than was folic acid in lowering tHcy (P < 0.05). At 24 wk, the increases in plasma folate and RCF concentrations did not differ significantly between the 2 supplemented groups.”
Am J Clin Nutr 2003 · PMID 12600857
“We observed only a significant decrease of total serum HCY in the L-5-MTHF group during the study period (at week 0: 15+/-7.7 microM; after 8 weeks treatment: 9.41+/-2.6 microM, P<0.001).”
Eur J Clin Nutr 2007 · PMID 17522618
“These findings suggest differential effects of l-5-MTHF compared with folic acid supplementation on blood folate concentrations but no differences on plasma total homocysteine lowering in Malaysian women.”
J Nutr 2018 · PMID 29878267
“Supplementation with [6 S]-5-MTHF was slightly less effective, but not significantly, in Hcy lowering than FA (p = .243 between the groups), that is, by 7.8% and 13.4%, respectively. The [6 S]-5-MTHF was shown to be an adequate alternative to FA in reducing Hcy concentrations.”
Int J Food Sci Nutr 2017 · PMID 28460586

Research describes the active mechanism and is not a claim about this specific product.

Educational information, not medical advice. This product is not intended to diagnose, treat, cure, or prevent any disease. Talk to your physician before starting any supplement or device, especially if pregnant, nursing, or taking medication.

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FAQ

Does L-Methylfolate (5-MTHF) have scientific evidence?

Yes — its active compound is linked to 21 peer-reviewed studies, summarized and cited above.

Where can I buy L-Methylfolate (5-MTHF)?

You can buy it on Amazon via the link above; Magellan earns a small affiliate commission at no extra cost to you, which does not affect the evidence grade.

Is L-Methylfolate (5-MTHF) safe?

Dietary supplements and devices are generally well tolerated but are not a substitute for medical care. Talk to your physician before starting, especially if you take medication or have a health condition.