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Study summary · Brain, cognition & mood

Does dietary luteolin reduce microglia activity in the brain of senescent mice?

In one paragraph

Luteolin Capsules, an animal study (Rejuvenation Res 2016, PMID 26918466) — in saline-treated aged mice fed luteolin, these proportions were reduced by nearly half to 12% for MHC class II, 13% for IL-1β, and 12% for IL-6. The study did not investigate the effects of luteolin on LPS-treated aged mice for IL-1β and IL-6, as the reduction was not significant in this group.

Source: Rejuvenation Res 2016, PMID 26918466 ↗ · Research map · How Magellan grades evidence · evidence confidence: high

Rejuvenation Res · 2016 · PMID 26918466 · DOI 10.1089/rej.2015.1708

Plain-English summary written and published by Magellan Longevity · medical review by Gabriel Radu, DO (physiatrist, NPI 1376861765). We summarize what the paper reported — we did not run this study.

The takeaway

Dietary luteolin reduced age-related proinflammatory microglia activity in the brains of senescent mice.

The question

Does dietary luteolin reduce microglia activity in the brain of senescent mice?

What they tested

Feeding aged mice a diet with luteolin would reduce microglia activity.

How they did it

Adult (3-6 months) and aged (22-24 months) mice were fed either a control diet (AIN-93M) or a diet supplemented with luteolin (6 g/kg) for 4 weeks. They were then injected with either saline or lipopolysaccharide (LPS). Microglia were isolated and stained for MHC class II, IL-1β, and IL-6 for flow cytometry.

What they found

In saline-treated mice on a control diet, aging increased the proportion of microglia staining for MHC class II (from <3% in adults to 23% in aged), IL-1β (from <2% in adults to 25% in aged), and IL-6 (from <2% in adults to 25% in aged). In saline-treated aged mice fed luteolin, these proportions were reduced by nearly half to 12% for MHC class II, 13% for IL-1β, and 12% for IL-6. Luteolin also significantly reduced IL-1β and IL-6 staining in LPS-treated adult mice, but not in aged mice.

Proportion of Proinflammatory Microglia in Aged Mice
Values shown: Proportion of Microglia (%)
Aged Control - MHC class II23
Aged Luteolin - MHC class II12
Aged Control - IL-1β25
Aged Luteolin - IL-1β13
Aged Control - IL-625
Aged Luteolin - IL-612

The chart shows the percentage of microglia staining for proinflammatory markers in aged mice fed a control diet versus those fed a luteolin-supplemented diet.

What it means

A diet supplemented with luteolin inhibited brain microglia activity during aging and also inhibited activation by LPS in adult mice. Luteolin may inhibit neuroinflammation and improve cognition in healthy aged individuals by constraining brain microglia.

Limitations

The study did not investigate the effects of luteolin on LPS-treated aged mice for IL-1β and IL-6, as the reduction was not significant in this group.

“a diet supplemented with luteolin inhibited brain microglia activity during aging”— from the published abstract, Rejuvenation Res 2016

The paper at a glance

TitleDietary Luteolin Reduces Proinflammatory Microglia in the Brain of Senescent Mice
JournalRejuvenation Res
Year2016
PMID26918466 ↗
DOI10.1089/rej.2015.1708 ↗
TopicBrain, cognition & mood

Read the source: PubMed record (authors, abstract, full citation) ↗ · Publisher via doi.org ↗

Where Magellan uses this paper

This citation sits behind the evidence grade on the pages below. Grades are set from the research and are independent of affiliate commissions.

Luteolin Capsules

Preclinical studies consistently show that luteolin reduces neuroinflammation and can ameliorate memory and learning deficits in rodent models of Alzheimer's disease, cerebral hypoperfusion and LPS-induced cognitive impairment, in part by inhibiting ER stress and cytokine release.

Luteolin Capsules

Luteolin is a naturally occurring flavone (a class of flavonoid) found in celery, parsley, green…

Check price on Amazon ↗

Research describes the compound or intervention studied. It is not a claim about any specific product.

Cite this page

These citations point at this summary. To cite the original paper with its full author list, use the PubMed record or doi.org.

APA
Magellan Longevity. (2026). Does dietary luteolin reduce microglia activity in the brain of senescent mice? [Plain-English summary of Rejuvenation Res 2016, PMID 26918466, DOI 10.1089/rej.2015.1708]. Magellan Longevity. https://magellanlongevity.com/study/d26918466.html
BibTeX
@misc{magellan_d26918466, title = {Does dietary luteolin reduce microglia activity in the brain of senescent mice?}, author = {{Magellan Longevity}}, year = {2026}, howpublished = {\url{https://magellanlongevity.com/study/d26918466.html}}, note = {Plain-English summary of PubMed PMID 26918466; DOI 10.1089/rej.2015.1708; Rejuvenation Res 2016. Reviewed by Gabriel Radu, DO}, urldate = {2026-08-11} }
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How this summary was made. Every section above restates what the published abstract of PMID 26918466 reports — the question, the design, the numbers, the authors’ own conclusion and their stated limitations. We do not add claims the paper did not make, and we keep negative and no-effect findings in. Magellan’s evidence grades are set from research like this and never from affiliate commissions.

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