Does hesperidin 2S supplementation improve endothelial function in overweight individuals?
Hesperidin Citrus Flavonoid, a randomized, double-blind, placebo-controlled trial in 68 overweight individuals (Am J Clin Nutr 2016, PMID 27797708) — no significant change in fasting or postprandial FMD was observed in the total study population after 6 weeks of hesperidin intake.
Source: Am J Clin Nutr 2016, PMID 27797708 ↗ · Research map · How Magellan grades evidence · evidence confidence: high
Am J Clin Nutr · 2016 · PMID 27797708 · DOI 10.3945/ajcn.116.136960
Hesperidin 2S may offer cardiovascular benefits by reducing adhesion molecules and blood pressure, particularly in overweight individuals with a relatively healthy endothelium.
The question
Does hesperidin 2S supplementation improve endothelial function in overweight individuals?
What they tested
Hesperidin, a citrus flavonoid, may exert beneficial effects on endothelial function due to its antioxidant and other biological properties.
How they did it
This was a randomized, double-blind, placebo-controlled study where 68 overweight individuals received either 450 mg/d of hesperidin 2S or a placebo for 6 weeks. Researchers assessed flow-mediated dilation (FMD), soluble adhesion molecules (sVCAM-1, sICAM-1, sP-selectin), systolic blood pressure (SBP), and diastolic blood pressure (DBP) at baseline and after 6 weeks. Acute endothelial dysfunction was induced by a high-fat meal, with FMD and adhesion molecules measured postprandially. An exploratory analysis was also conducted in subjects with baseline FMD "3%.
What they found
No significant change in fasting or postprandial FMD was observed in the total study population after 6 weeks of hesperidin intake. However, there was a trend for a reduction in sVCAM-1, sICAM-1, sP-selectin, SBP, and DBP after 6 weeks of hesperidin treatment. In the exploratory analysis of subjects with baseline FMD ≥3%, hesperidin protected individuals from postprandial endothelial dysfunction (P = 0.050) and significantly downregulated sVCAM-1 and sICAM-1 (all P ≥0.030).
What it means
Six weeks of hesperidin 2S consumption did not improve basal or postprandial FMD in the overall study population, but showed a tendency to reduce adhesion molecules and blood pressure. In overweight and obese individuals with a relatively healthy endothelium (baseline FMD ≥3%), hesperidin 2S improved endothelial dysfunction after a high-fat meal and reduced adhesion molecules, suggesting cardiovascular health benefits.
Limitations
The results reported were not adjusted for multiplicity. The significant findings regarding FMD and adhesion molecules were from an exploratory analysis in a subgroup of participants.
The paper at a glance
| Magellan’s evidence label | lowers adhesion molecules, BP (our label for this citation — see PubMed for the paper’s own title and authors) |
|---|---|
| Journal | Am J Clin Nutr |
| Year | 2016 |
| PMID | 27797708 ↗ |
| DOI | 10.3945/ajcn.116.136960 ↗ |
| Topic | Cardiovascular & vascular aging |
Read the source: PubMed record (authors, abstract, full citation) ↗ · Publisher via doi.org ↗
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Hesperidin Citrus Flavonoid
In human trials, hesperidin or hesperidin-rich orange juice has improved endothelial function and postprandial microvascular reactivity, modestly lowered blood pressure in some studies — including a 159-person trial showing dose-dependent systolic and pulse-pressure reductions with sustained intake — and reduced…
Hesperidin is a flavanone glycoside (a citrus bioflavonoid) found abundantly in oranges, lemons, and…
Research describes the compound or intervention studied. It is not a claim about any specific product.
Molecules & mechanisms in this paper
Each of these is named in the paper’s own words above. Open the monograph for the full mechanism and its other citations.
Cite this page
These citations point at this summary. To cite the original paper with its full author list, use the PubMed record or doi.org.
Magellan Longevity. (2026). Does hesperidin 2S supplementation improve endothelial function in overweight individuals? [Plain-English summary of Am J Clin Nutr 2016, PMID 27797708, DOI 10.3945/ajcn.116.136960]. Magellan Longevity. https://magellanlongevity.com/study/s113.html@misc{magellan_s113,
title = {Does hesperidin 2S supplementation improve endothelial function in overweight individuals?},
author = {{Magellan Longevity}},
year = {2026},
howpublished = {\url{https://magellanlongevity.com/study/s113.html}},
note = {Plain-English summary of PubMed PMID 27797708; DOI 10.3945/ajcn.116.136960; Am J Clin Nutr 2016. Reviewed by Gabriel Radu, DO},
urldate = {2026-08-11}
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