Does long-term nicotinamide mononucleotide (NMN) supplementation influence biochemical and metabolic health parameters, including NAD metabolism and arterial stiffness, in healthy middle-aged adults?
Nicotinamide Mononucleotide, a randomized, double-blind, placebo-controlled trial in 36 healthy middle-aged participants (Sci Rep 2023, PMID 36797393) — NMN supplementation at 250 mg/day was well tolerated with no adverse events. The study noted that while arterial stiffness tended to decrease, no significant difference was found between the two groups, suggesting a potential limitation in statistical power or effect size for this specific outcome.
Source: Sci Rep 2023, PMID 36797393 ↗ · Research map · How Magellan grades evidence · evidence confidence: high
Sci Rep · 2023 · PMID 36797393 · DOI 10.1038/s41598-023-29787-3
NMN supplementation safely increased NAD metabolism in healthy middle-aged adults and showed a non-significant trend towards reducing arterial stiffness.
The question
Does long-term nicotinamide mononucleotide (NMN) supplementation influence biochemical and metabolic health parameters, including NAD metabolism and arterial stiffness, in healthy middle-aged adults?
What they tested
The study implicitly hypothesized that NMN supplementation would prevent the reduction of NAD levels and potentially alleviate aging-related diseases, as observed in animal studies.
How they did it
A 12-week randomized, double-blind, placebo-controlled, parallel-group clinical trial was conducted with 36 healthy middle-aged participants. Participants received either 125 mg NMN or placebo twice daily.
What they found
Serum nicotinamide levels were significantly higher in the NMN group compared to the placebo group. Pulse wave velocity, an indicator of arterial stiffness, tended to decrease in the NMN group, but this difference was not statistically significant between the two groups. NMN supplementation at 250 mg/day was well tolerated with no adverse events.
| NMN Dose per Capsule | 125 | |
|---|---|---|
| Total Daily NMN Dose | 250 |
Participants received 125 mg of NMN per capsule, taken twice daily for a total daily dose of 250 mg.
What it means
NMN supplementation safely and effectively elevated NAD metabolism in healthy middle-aged adults. While NMN showed potential in alleviating arterial stiffness, this effect was not statistically significant in this study.
Limitations
The study noted that while arterial stiffness tended to decrease, no significant difference was found between the two groups, suggesting a potential limitation in statistical power or effect size for this specific outcome.
The paper at a glance
| Title | NAD metabolism and arterial stiffness after long-term nicotinamide mononucleotide supplementation: a randomized controlled trial |
|---|---|
| Journal | Sci Rep |
| Year | 2023 |
| PMID | 36797393 ↗ |
| DOI | 10.1038/s41598-023-29787-3 ↗ |
| Topic | NAD⁺, sirtuins & cellular energy |
Read the source: PubMed record (authors, abstract, full citation) ↗ · Publisher via doi.org ↗
Where Magellan uses this paper
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Nicotinamide Mononucleotide (NMN)
In randomized controlled trials, NMN reliably increases blood NAD+ levels and has generally been well tolerated at oral doses up to roughly 900-1250 mg/day, with some trials reporting modest improvements in skeletal-muscle insulin sensitivity, walking speed, aerobic capacity, sleep quality, and diastolic blood…
NAD⁺ Activation Stack (NMN + Resveratrol + Pterostilbene)
Sirtuins require NAD⁺ to deacetylate their targets, so raising NAD⁺ availability and stimulating sirtuin enzymes are complementary strategies. This pairing mirrors the molecular targets engaged by caloric restriction — the most reproducible lifespan-extending intervention in laboratory animals — which is why combined…
Nicotinamide mononucleotide (NMN) is a naturally occurring nucleotide and a direct biosynthetic…
Nicotinamide mononucleotide (NMN) is a naturally occurring nucleotide and a direct biosynthetic…
Nicotinamide mononucleotide (NMN) is a naturally occurring nucleotide and a direct biosynthetic…
Nicotinamide mononucleotide (NMN) is a naturally occurring nucleotide and a direct biosynthetic…
Nicotinamide mononucleotide (NMN) is a naturally occurring nucleotide and a direct biosynthetic…
Nicotinamide mononucleotide (NMN) is a naturally occurring nucleotide and a direct biosynthetic…
Research describes the compound or intervention studied. It is not a claim about any specific product.
Molecules & mechanisms in this paper
Each of these is named in the paper’s own words above. Open the monograph for the full mechanism and its other citations.
Cite this page
These citations point at this summary. To cite the original paper with its full author list, use the PubMed record or doi.org.
Magellan Longevity. (2026). Does long-term nicotinamide mononucleotide (NMN) supplementation influence biochemical and metabolic health parameters, including NAD metabolism and arterial stiffness, in healthy middle-aged adults? [Plain-English summary of Sci Rep 2023, PMID 36797393, DOI 10.1038/s41598-023-29787-3]. Magellan Longevity. https://magellanlongevity.com/study/x1.html@misc{magellan_x1,
title = {Does long-term nicotinamide mononucleotide (NMN) supplementation influence biochemical and metabolic health parameters, including NAD metabolism and arterial stiffness, in healthy middle-aged adults?},
author = {{Magellan Longevity}},
year = {2026},
howpublished = {\url{https://magellanlongevity.com/study/x1.html}},
note = {Plain-English summary of PubMed PMID 36797393; DOI 10.1038/s41598-023-29787-3; Sci Rep 2023. Reviewed by Gabriel Radu, DO},
urldate = {2026-08-11}
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